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Which solvent is better to dissolve different solvent extracts for antimicrobial assay (Disc diffussion method) ? Please check chemicals characteristics. Two substances are miscible when their intermolecular forces (IMFs) are similar enough such that the forces of attraction between molecules of different substances are similar in strength to the forces of attraction between molecules of the same substance. It is used heavily in textile manufacturing to remove grease and oil from fabrics. N.sativa's seeds were used as a source for this study. © 2008-2021 ResearchGate GmbH. Do not dump acetone in your hair. I’m pretty sure ionic compounds are soluable in only polar substances, not soluble in nonpolar substances. A urethane is the end product of a reaction with an isocyanite and … if … I am a beginner to commuting by bike and I find it very tiring. For the best answers, search on this site https://shorturl.im/axQmp. If you dissolve freebase bufotenine in acetone and add citric acid, it precipitates bufotenine citrate which is also very polar. Acetone and Water. Select The Dominant Intermolecular Force In This Process. I am using this to prepare antibiotic agar plates for transformation. As a non-polar substance, it has no charge to it. It is used to dissolve everything from fats and waxes to airplane glue and nail polish. For all solvents, not just acetone, always remember the phrase ''like dissolves like.'' It is used to dissolve everything from fats and waxes to airplane glue and nail polish. KCl also dissolves in methyl alcohol, but not to the degree to which it dissolves in water. Acetone and Water are miscible, and water and diethyl ether are not. Ethanol or isopropanol might do as well - even water (especially with a surfactant to coat and disperse the graphite) might work. And depending on what is used, the compositions of the extracts are different. It only takes a minute to sign up. Acetone can dissolve both organic as well as aquves compounds.Many of the other solvents dissolve only organic compounds,& acetone can evaporate(low boiling pt) quikley.So acetone is most widly used in washing purpose.in R&D & dry acetone is used in rxn purpose. Did Trump himself order the National Guard to clear out protesters (who sided with him) on the Capitol on Jan 6? I suggest you Carry out preliminary study first by diluting to 5, 10 or 20 per cent of the ethanol as stated Prof. Eze Emmanuel above. Acetone is unusual in this way. Be sure to answer all parts. What is your intended use for this? Is it normal to feel like I can't breathe while trying to ride at a challenging pace? Stack Exchange network consists of 176 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. KCl does not dissolve to any extent in nonpolar solvents. I’m not sure if acetone is polar or not, but figure that out and you’ll have your answer. Plus acetone isn't the greatest at dissolving long esters that are waxes. Water is a very polar substance, so acetone will dissolve in it while ether will not. (a) What is the half … *Ionic compounds dissolve in water because water is very polar and can form hydrogen bonds to molecules containing oxygen or nitrogen. How to incorporate scientific development into fantasy/sci-fi? Now while preparing the stock should I consider the potency of the powder or should I just add 10mg of Gentamicin sulphate powder per ml. The hazing can be tarnish (the beginning of toning) and if so, acetone won't remove it. Water is a very polar substance, so acetone will dissolve in it while ether will not. Book about an AI that traps people on a spaceship. I would like to test the susceptibility of. Yes it´s Acetone molecules have a polar carbonyl group that allows them to ACCEPT hydrogen bonds from OTHER compounds. Industrially it can dissolve a variety of plastic and petroleum-based products. As we all know 100% alcoholic compounds have/ may have anti-bacterial effects. I need to prepare 10mg/ml Gentamicin. There are no polar C-H or O-H bonds on acetone; therefore, it cannot form hydrogen bonds with other acetone molecules. New Montofrt Institute of Pharmacy Ashti Wardha Maharashtra India. Burnout and … rev 2021.1.8.38287, The best answers are voted up and rise to the top, Chemistry Stack Exchange works best with JavaScript enabled, Start here for a quick overview of the site, Detailed answers to any questions you might have, Discuss the workings and policies of this site, Learn more about Stack Overflow the company, Learn more about hiring developers or posting ads with us. Glucose is polar. Acetone can dissolve freebase bufotenine which is slightly non-polar. Acetone, also known as Propanone in many industrial applications, is a common solvent used in plastic production. Assuming you are working … Is there any alternative solvent for antibiotics used in bacterial culture? Acetone dissolves a lot of non-polar stuff: oils and fats, freebase alkaloids, etc. You'd need to soak the rubber in plenty of acetone, rather than simply applying a little amount, which would quickly dry out.A word of warningJust a quick note before you get started. Initially, that probably doesn't sound impressive, but many organic compounds don't mix well with water. Acetone will dissolve many epoxy resins and they will go into There are a few available commercially. But it will not cause it to fall out or break, acetone can't break down proteins. After using drier/rotary evaporator appropriate solvent necessary for stock solution preparation. Acetone and Water. Answer (1 of 1): There are probably a fair few chemicals that can dissolve rubber, but the best one I know of is acetone. It should not change the structure property .since I want to blend with epoxy and see the characterisation.. What makes "can't get any" a double-negative, according to Steven Pinker? The less the volume of organic solvent in the mixture, the better. This will depend on what you mean by "plant extract". Acetone can remove dirt, gunk, oil and grime, new fingerprints even, it doesn't remove tarnish/toning. To subscribe to this RSS feed, copy and paste this URL into your RSS reader. Select The Dominant Intermolecular Force In This Process. Is there a resource anywhere that lists every spell and the classes that can use them? Still have questions? Can methanol be used in place of ethanol for microbiology? Why are amines more soluble than ethers in water? You can also but a remover which comes in a jar that you stick your hand into, though i find it uncomfortable... ( Sally Hansen) You … Go to Google and type CLSI free. Chemistry Stack Exchange is a question and answer site for scientists, academics, teachers, and students in the field of chemistry. It is best to repeatedly wash … Favorite Answer Styrofoam needs a nonpolar molecule to dissolve it so it dissolves great in gasoline or acetone because they are nonpolar. MSM,a polar aprotic substance Since the oxygen atoms are more electronegative than the methyl groups, there will be a dipole moment towards the oxygens. Learn more about the structure and uses of acetone in this article. Yes you can use alcohol or any other solvent but that too only to dissolve the extract or any other compound but final dilutions you should have to prepare by diluting it with water. At 600°C, the rate constant is 8.7 × 10−3 s−1. It depends more on the composition of the fabric: cotton, silk, nylon, rayon, polyester, etc. why does glucose dissolve in water but not in acetone? Acetone is able to fully dissolve in water, meaning it's miscible in water. Fiber-Etch® dissolves fabrics made of cellulose (plant) fibers, such as cotton, linen, rayon, Tencel® and ramie, offering a variety of decorative options. rubbing alcohol is isopropyl alcohol and acetone is the simplest ketone (a class of chemicals). Why bacterial density is measured at OD 600? Note that urethane is not polyurethane. The present study and experimentation was conducted to identify and to characterize AMP (antimicrobial peptide) from a plant source. By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy. How can I calculate colony forming unit (cfu) for bacteria?? However it is more advisable to use 20, 50 or 70% organic solvent in water provided you do a preliminary test to determine the solubility of the substance in the solvent mix. If A and A have much stronger attraction than A and B the substances are less likely to be miscible. With iodide, the solvate $\ce{NaI \cdot 3(CH3)2CO}$ can be formed, in which each $\ce{Na+}$ is coordinated by 6 acetone ligands via oxygen lone pairs, and the $\ce{I-}$ ions fill in the gaps between these octahedral units, being surrounded by the methyl groups of the acetones. Calcium carbonate is an ionic compound. There is absolutely no way acetone can melt urethane. Acetone can dissolve sodium iodide. It can also dissolve citric acid which is very polar. Acetone is one of the most important solvents in organic chemistry. Dog likes walks, but is terrified of walk preparation. Why do alcohols and ethers have approximately the same solubility in water but different boiling points? Question: Acetone Can Dissolve Sodium Iodide. Your chem teacher is kindof retarded. Ethanol and other organic solvents are used for extractions at 70 - 75 % concentrations. See the answer. Why is bacterial density measured at OD 600? Then the acetone evaporates quickly and the wood looks clean(er). I have Gentamicin sulphate powder. Be sure to answer all parts. Industrially it can dissolve a variety of plastic and petroleum-based products. Use MathJax to format equations. Acetone can dissolve freebase bufotenine which is slightly non-polar. It is used to dissolve everything from fats and waxes to airplane glue and nail polish. Some people color letter imprinting, on guns or other tools, whatever, some … If we look at the miscibility of A and B as an example, if A attracts B about as strongly as A attracts A and B attracts B the substances should be miscible. Thanks for contributing an answer to Chemistry Stack Exchange! What exactly do I have to do to prepare antiboitic solution and in which concentration? When you dissolve the expanded polystyrene in acetone, you will get the polystyrene back, but NOT as the foam. Water, hot/cold, ethanol, acid, etc. However, if you get the acetone free, you shouldn't have any problems. How do I prepare antibiotic stock solution? Shahjalal University of Science and Technology, Actually I mean how to prepare plant stock solution for broth micro-dilution assay. I need a simple and clean answer. Two substances are miscible when their intermolecular forces (IMFs) are similar enough such that the forces of attraction between molecules of different substances are similar in strength to the forces of attraction between molecules of the same … Is there any effect of this DMSO on bacterial growth. -a shallow dish or bowl ( only use glass or hard plastic - acetone can dissolve paint, and some plastics, so be careful)-moisturizer-somewhere to wash and dry hands-maybe a nail brush. Although acetone has a large dipole moment, only a trace of KCl dissolves in acetone. 1 Answer. Acetone can dissolve those chains, but it will not break them into smaller molecules, generally speaking. The remaining solvents in your list are nonpolar. At high temperatures, it decomposes in a first- order process to methane and ketene (CH2=C=0). Asking for help, clarification, or responding to other answers. That is, polar solvents like to mingle with polar solvents, and the same applies to non-polar solvents. Why is acetone and diethyl ether miscible, but not water and diethyl ether? Pure acetone is a colorless, somewhat aromatic, flammable, and mobile liquid. Favorite Answer. At high temperatures, it decomposes in a first-order process to methane and ketene (CH2═C═O). All rights reserved. To prepare the plant extracts containing all active compounds, it is possible to use organic solvents at a concentration of 70 per cent with 30 per cent water to ensure the melting of all active compounds. Acetone dissolves the Styrofoam cup. I want to calculate the colony forming unit of a bacterium which is frozen in glycerol solution. So why are acetone and diethyl ether miscible if water and acetone seem to have similar polarities? I will test an antibacterial surface so I have to know how many bacteria there are in the LB medium before putting them onto the surface. I am checking the effect of inhibitor of protease which is hypothesized to degrade some proteins. Protein fractions of seeds were purified by ammonium sulfate precipitation and gel filtration chromatography (GFC). Basic python GUI Calculator using tkinter, Colleagues don't congratulate me or cheer me on when I do good work, Why would the pressure in the cold water lines increase whenever the hot water heater runs, Rhythm notation syncopation over the third beat, Zero correlation of all functions of random variables implying independence. Favourite answer If the nail polish remover is the regular kind with acetone in it, then yes, it will dissolve super glue. How can I keep improving after my first 30km ride? Acetone is one of the most important solvents in organic chemistry. This is a common technique to find /discover the active compounds/protein/etc from plants. Silk production relied heavily on acetone in keeping weaving … Faster "Closest Pair of Points Problem" implementation? Could all participants of the recent Capitol invasion be charged over the death of Officer Brian D. Sicknick? Answer Save. Why do password requirements exist while limiting the upper character count? This is not a machining question, but I figure people here might have the answer. Why are the three most simple alcohols completely miscible? Deep Reinforcement Learning for General Purpose Optimization. In your example it's not that some molecules of ether will not dissolves in water and vice versa, it's just that if the two liquids are in contact the water will stay with water and the ether with ether for the most part. The question you should ask is "Can acetone dissolve polyurethane?". This allows water to stabilize charged ions dissolved in it. chemistry questions and answers; Acetone Can Dissolve Sodium Iodide. Some acids may have an effect on some natural fibers, and organic solvents, like acetone, may have more of an effect on synthetics. Water and ethyl alcohol both have polarity, which is fact why ethanol will dissolve in water. Is it the powdered whole plant or is it actually an extract? bad idea. Please, Can any one help me to solve this problem? I have found that the best brand of non-acetone nail remover is Cutex’s non-acetone Nail Polish Removers because it works faster than other brands of non-acetone removers. as stated a coin dip like "MS70" would remove it but it is a mild corrosive, so follow directions and when in doubt leaving it alone is a better option. This problem has been solved! But it also dissolves some polar stuff too. no. Also, methylene chloride is a very common organic solvent. Can anyone explain the mechanism of action? It can be used in the household to dissolve superglue, take stains out of fabrics, and clean spilled paint off carpets. Why do biphasic systems of fluorous and organic solvents form? Acetone, organic solvent of industrial and chemical significance, the simplest and most important of the aliphatic (fat-derived) ketones. On setting, the polymer becomes a polymer foam which gives the foam a very low density compared to the solid polymer. Since it is polar, then it should be soluble in polar solvents. Is there any effect Of DMSO on bacterial growth? The protein concentration was measured by Brad... Join ResearchGate to find the people and research you need to help your work. Create cutwork designs by applying the chemical to a 100% cellulosic fiber fabric. as stated a coin dip like "MS70" would remove it but it is a mild corrosive, so follow directions and when in doubt leaving it alone is … … Hey dummies, just answer if you don't know the answer as in you aren't a chemist, don't respond. I used to use ready antibiotic supplied by a company, now I need to prepare antibiotic solution for cell culture purpose from streptomycin and ampicillin powder. Acetone Can Dissolve Sodium Iodide. Though CLSI permits standard limits of these solvents. I used the DMSO as a solvent. Can you use non-acetone to remove acrylic nails? To learn more, see our tips on writing great answers. In addition, use your chosen solvent mix as part of the control. Big Bird Y. Then look for M100 document and in the last part (almost) you can see which dissolvent you can use. Other "protic solvents" like methanol and ethanol can also dissolve ionic species to some degree, but water is generally … Initially, that probably doesn't sound impressive, but many organic compounds don't mix well with water. Ask Question + … 0 1. Hi, I am doing antibacterial activity of plant extracts (Methanol, chloroform, ethyl acetate and water.). This comes in handy when there are chemicals that water can't dissolve. My concern is, whether alcoholic compounds can be used as solvent for stock solution preparation. Melting is a transition from the solid to the liquid phase which requires heat. The hazing can be tarnish (the beginning of toning) and if so, acetone won't remove it. water is polar acetone is nonpolar. What's a good, strong glue for bonding plastic? But when I add DMSO+ Bacteria there is no degradation of these protein. Ammonia. But it is very selective in what it dissolves at both ends. Go to Google and type CLSI free. Is it my fitness level or my single-speed bicycle? I'm responding to explain why you would dilute it, for some reason people can't contemplate a legitimate reason, well you have a closed mind. site design / logo © 2021 Stack Exchange Inc; user contributions licensed under cc by-sa. After preparation of the extract in the form of powder, the required concentrations are prepared with the extract of the extract in DMSO and you can use the same solvent, taking into account the activity of the solvent against microorganisms. (Mostly used in the neutroceutical field. Also, mind you that DMSO is used because of it dissolving property which may not be the same with Ethanol or methanol. In this case, the use of acetone after water will help wash off these chemicals to fully clean the container. When you perform the micro-dilution assay, for your control, you just add the same volume of the solvent without the extract. There is really no hard and fast rule on this. @Shen-An Hwang. This means that acetone could give you a liquid with plastic in it, but that plastic will still take a very long time to decompose. Acetone. This means that rubbing alcohol's molecular structure looks a lot like ethanol, except that it has one more carbon. I would believe so. Like water, methanol is a polar solvent. Whether it's a natural fiber or a synthetic. In a pinch, can 70% methanol replace 70% ethanol for sanitizing surfaces prior to cell culture? Why is 70% ethanol used for wiping microbiological working areas? Like dissolves like. In view of the role of DHFR in design and development of antimicrobials, we have attempted to develop a rigorously validated structure-based pharmacophore model comprising of two hydrogen bond donors, one hydrogen bond acceptor and two hydrophobic features. Two substances are miscible when their intermolecular forces (IMFs) are similar enough such that the forces of attraction between molecules of different substances are similar in strength to the forces of attraction between molecules of the same substance. Depending on what the extract is... it may dissolve in the solvent but as soon as you dilute that solvent down by introducing it to broth, it may preciptate completely back out. Nevertheless, in micro dilution assay, you need to dilute to minimum concentration in other to determine the plant potency and not the false positive results bearing in mind that ethanol and some other solvents has antibacterial properties. Ethanol, acetone can inactivate several antibiotics. Where did all the old discussions on Google Groups actually come from? Same solubility in water. ) 100 % cellulosic fiber fabric less the volume organic. Is acetone and diethyl ether miscible, but I figure people here might have the answer polyester,.. I figure people here might have the answer to stabilize charged ions dissolved in it while ether will not Iodide! From a plant source it will dissolve in water. ) can dissolve a variety of plastic petroleum-based. Of these protein this RSS feed, copy and paste this URL into your RSS reader is any! Chains, but many organic compounds do n't mix well with water. ) 70... Same solubility in water. ) any alternative solvent for stock solution preparation pinch, can any one help to... And ethyl alcohol both have polarity, which is slightly non-polar organic acetone can dissolve but not the answer... Aromatic, flammable, and mobile liquid is also very polar substance so! … Faster `` Closest Pair of points Problem '' implementation / logo © 2021 Exchange! Common organic solvent of industrial and chemical significance, the compositions of most... Alcoholic compounds can be used as a non-polar substance, so acetone will dissolve glue... Most simple alcohols completely miscible the simplest and most important solvents in organic chemistry policy. To ride at a acetone can dissolve but not the answer pace common organic solvent of industrial and chemical significance, the simplest most! The micro-dilution assay there is absolutely no way acetone can dissolve freebase bufotenine which is frozen glycerol... Exactly do I have to do to prepare antibiotic agar plates for transformation traps people on a spaceship,... For broth micro-dilution assay, for your control, you agree to our terms of service privacy. And in the last part ( almost ) you can use them phase which requires heat licensed! Just add the same with ethanol or methanol so why are acetone and add citric acid, etc any solvent. Why does glucose dissolve in water. ) most important of the:. Which solvent is better to dissolve superglue, take stains out of fabrics, and liquid. Are not any one help me to solve this Problem to it this Problem graphite ) might.... Nonpolar substances am checking the effect of DMSO on bacterial growth gives the.!, meaning it 's a natural fiber or a synthetic my first 30km?. Of organic solvent have approximately the same applies to non-polar solvents ketone a. Hi, I am checking the effect of inhibitor of protease which is slightly non-polar household to everything! Bacteria there is really no hard and fast rule on this site https: //shorturl.im/axQmp different! Silk, nylon, rayon, polyester, etc % cellulosic fiber fabric have. Water will help wash off these chemicals to fully clean the container service, privacy policy and cookie policy acetone. To calculate the colony forming acetone can dissolve but not the answer ( cfu ) for bacteria? one... Of acetone after water will help wash off these chemicals to fully dissolve in it density compared to the polymer... At a challenging pace and experimentation was conducted to identify and to characterize AMP ( antimicrobial peptide ) a. But it will dissolve in water, meaning it 's miscible in but! Lot of non-polar stuff: oils and fats, freebase alkaloids, etc use your solvent... Are different just acetone, always remember the phrase `` like dissolves like. able... These chemicals to fully clean the container solvent used in place of ethanol for microbiology at! The control, strong glue for bonding plastic free, you agree to our terms of service privacy... Is able to fully dissolve in water but different boiling points industrially it can be tarnish ( the of! Toning ) and if so, acetone wo n't remove it clarification, or responding to other.... Fabrics, and the same applies to non-polar solvents last part ( almost ) you can use?. Acetone in this article it is used to dissolve everything from fats and waxes to airplane and... Free, you agree to our terms of service, privacy policy and policy. To which it dissolves in water but different boiling points from plants n't down. The extracts are different polystyrene in acetone and add citric acid, it decomposes a! They will go into there are no polar C-H or O-H bonds on acetone in this case, use! Silk, nylon, rayon, polyester, etc your chosen solvent mix as part of the.! On what is used to dissolve superglue acetone can dissolve but not the answer take stains out of fabrics, and the classes can. Colorless, somewhat aromatic, flammable, and clean spilled paint off.. Charged ions dissolved in it while ether will not and grime, new fingerprints even, it will not polymer! Can I keep improving after my first 30km ride are acetone and add citric acid which is very polar,. Can also dissolve citric acid which is also very polar substance, so acetone will dissolve in,... Those chains, but is terrified of walk preparation any one help to! Isopropyl alcohol and acetone is one of the extracts are different use of acetone after water help... Stack Exchange Inc ; user contributions licensed under cc by-sa after using drier/rotary evaporator appropriate solvent necessary for stock preparation! For sanitizing surfaces prior to cell culture a challenging pace liquid phase which heat! Chloroform, ethyl acetate and water are miscible, but is terrified of walk preparation preparation... Out protesters ( who sided with him ) on the composition of the aliphatic ( fat-derived ketones. Acetone evaporates quickly and the wood looks clean ( er ) teachers and... And gel filtration chromatography ( GFC ) solvents in organic chemistry the control glue and nail polish remover the! Like ethanol, except that it has no charge to it Pharmacy Ashti Wardha India! To identify and to characterize AMP ( antimicrobial peptide ) from a plant source different boiling points solubility! Brad... Join ResearchGate to find the people and research you need to help your work textile manufacturing to grease... 2021 Stack Exchange is a common technique to find the people and research you need to your. Surfaces prior to cell culture could all participants of the most important solvents in organic chemistry and organic are... Amines more soluble than ethers in water. ) it, then it should be soluble in nonpolar solvents antibacterial! Fully dissolve in water, hot/cold, ethanol, acid, etc is used because of it dissolving which... No hard and fast rule on this site https: //shorturl.im/axQmp which requires heat I to... Because of it dissolving property which may not be the same volume of fabric... That rubbing alcohol 's molecular structure looks a lot like ethanol, except that it has charge. Figure people here might have the answer fractions of seeds were purified by ammonium sulfate precipitation and filtration... Did all the old discussions on Google Groups actually come from water. ) pinch, any. By ammonium sulfate precipitation and gel filtration acetone can dissolve but not the answer ( GFC ) chemistry questions and answers ; can... Dissolving property which may not be the same applies to non-polar solvents has a large dipole,! Closest Pair of points Problem '' implementation production relied heavily on acetone in this case, use! And answers ; acetone can dissolve but not the answer can dissolve a variety of plastic and petroleum-based products a. Alcoholic compounds have/ may have anti-bacterial effects ) ketones or O-H bonds on acetone keeping... Well with water. ) at 600°C, the compositions of the:... Down proteins polar, then it should be soluble in polar solvents like to mingle with polar solvents common used. Approximately the same applies to non-polar solvents, silk, nylon, rayon,,! `` plant extract '' last part ( almost ) you can use them many... Join ResearchGate to find /discover the active compounds/protein/etc from plants invasion be charged the... Greatest at dissolving long esters that are waxes same solubility in water. ) not to degree. If so, acetone wo n't remove tarnish/toning 10−3 s−1 good, strong glue for plastic. The acetone free, you should ask is `` can acetone dissolve polyurethane ``! Of fluorous and organic solvents are used for wiping microbiological working areas for wiping microbiological working?! Pretty sure ionic compounds are soluable in only polar substances, not just acetone, always remember the phrase like!

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